Journal: Calcified tissue international
Article Title: Sclerostin Directly Stimulates Osteocyte Synthesis of Fibroblast Growth Factor-23.
doi: 10.1007/s00223-021-00823-6
Figure Lengend Snippet: Fig. 4 Effect of PTH and rhSCL on the IL-6/NF-κB pathway. IDG- SW3 cells differentiated for 5 weeks were treated with a rhPTH1-34 and b rhSCL for 24 h at the concentrations indicated and Il6 mRNA levels measured by real time RT-PCR. Il6 mRNA levels were also measured in a time course in response to c rhPTH1-34 (100 nM), and d rhSCL (50 ng/ml). Differentiated (5 wk) IDG-SW3 cells were pre-treated with NF-κβ signal inhibitors Bay11 (100 μM) or TPCK
Article Snippet: Differentiated IDG-SW3 cells were treated for the indicated time intervals with rhPTH1-34 (Tocris Bioscience) at concentrations between 1 and 100 nM or recombinant human sclerostin (rhSCL) (R&D Systems) at concentrations between 1 and 50 ng/ml [28, 29] for the time periods 1 3 indicated.
Techniques: Quantitative RT-PCR